Dataset Information

Plasma and urinary amino acid metabolomic profiling in patients with different levels of kidney function.

ABSTRACT:

Background and objectives

Patients with CKD display altered plasma amino acid profiles. This study estimated the association between the estimated GFR and urinary and plasma amino acid profiles in CKD patients.

Design, setting, participants, & measurements

Urine and plasma samples were taken from 52 patients with different stages of CKD, and plasma samples only were taken from 25 patients on maintenance hemodialysis. Metabolic profiling was performed by liquid chromatography coupled with tandem mass spectrometry after phenylisothiocyanate derivatization.

Results

Most plasma amino acid concentrations were decreased in hemodialysis patients, whereas proline, citrulline, asparagine, asymmetric dimethylarginine, and hydroxykynurenine levels were increased (P<0.05). Both plasma levels and urinary excretion of citrulline were higher in the group of patients with advanced CKD (CKD stages 2 and 3 versus CKD stages 4 and 5; in plasma: 35.9±16.3 versus 61.8±23.6 µmol/L, P<0.01; in urine: 1.0±1.2 versus 7.1±14.3 µmol/mol creatinine, P<0.001). Plasma asymmetric dimethylarginine levels were higher in advanced CKD (CKD stages 2 and 3, 0.57±0.29; CKD stages 4 and 5, 1.02±0.48, P<0.001), whereas urinary excretion was lower (2.37±0.93 versus 1.51±1.43, P<0.001). Multivariate analyses adjusting on estimated GFR, serum albumin, proteinuria, and other covariates revealed associations between diabetes and plasma citrulline (P=0.02) and between serum sodium and plasma asymmetric dimethylarginine (P=0.03). Plasma tyrosine to phenylalanine and valine to glycine ratios were lower in advanced CKD stages (P<0.01).

Conclusion

CKD patients have altered plasma and urinary amino acid profiles that are not corrected by dialysis. Depending on solutes, elevated plasma levels were associated with increased or decreased urinary excretion, depicting situations of uremic retention (asymmetric dimethylarginine) or systemic overproduction (citrulline). These results give some insight in the CKD-associated modifications of amino acid metabolism, which may help improve their handling.

SUBMITTER: Duranton F

PROVIDER: S-EPMC3878706 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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Publications

Plasma and urinary amino acid metabolomic profiling in patients with different levels of kidney function.

Duranton Flore F Lundin Ulrika U Gayrard Nathalie N Mischak Harald H Aparicio Michel M Mourad Georges G Daurès Jean-Pierre JP Weinberger Klaus M KM Argilés Angel A

Clinical journal of the American Society of Nephrology : CJASN 20131114 1

<h4>Background and objectives</h4>Patients with CKD display altered plasma amino acid profiles. This study estimated the association between the estimated GFR and urinary and plasma amino acid profiles in CKD patients.<h4>Design, setting, participants, & measurements</h4>Urine and plasma samples were taken from 52 patients with different stages of CKD, and plasma samples only were taken from 25 patients on maintenance hemodialysis. Metabolic profiling was performed by liquid chromatography coupl ...[more]

PMID: 24235289

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Dataset Information

Plasma and urinary amino acid metabolomic profiling in patients with different levels of kidney function.

Background and objectives

Design, setting, participants, & measurements

Results

Conclusion

Publications

Plasma and urinary amino acid metabolomic profiling in patients with different levels of kidney function.

Similar Datasets

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