Multiplexed MS/MS for improved data-independent acquisition.
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ABSTRACT:
SUBMITTER: Egertson JD
PROVIDER: S-EPMC3881977 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
Publications
Multiplexed MS/MS for improved data-independent acquisition.
Egertson Jarrett D JD Kuehn Andreas A Merrihew Gennifer E GE Bateman Nicholas W NW MacLean Brendan X BX Ting Ying S YS Canterbury Jesse D JD Marsh Donald M DM Kellmann Markus M Zabrouskov Vlad V Wu Christine C CC MacCoss Michael J MJ
Nature methods 20130623 8
In mass spectrometry-based proteomics, data-independent acquisition (DIA) strategies can acquire a single data set useful for both identification and quantification of detectable peptides in a complex mixture. However, DIA data are noisy owing to a typical five- to tenfold reduction in precursor selectivity compared to data obtained with data-dependent acquisition or selected reaction monitoring. We demonstrate a multiplexing strategy, MSX, for DIA analysis that increases precursor selectivity f ...[more]
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