Rescuing recombinant proteins by sequestration into the P22 VLP.
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ABSTRACT:
SUBMITTER: Patterson DP
PROVIDER: S-EPMC3882142 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Publications
Rescuing recombinant proteins by sequestration into the P22 VLP.
Patterson Dustin P DP LaFrance Benjamin B Douglas Trevor T
Chemical communications (Cambridge, England) 20131101 88
Here we report the use of a self-assembling protein cage to sequester and solubilize recombinant proteins which are usually trafficked to insoluble inclusion bodies. Our results suggest that protein cages can be used as novel vehicles to rescue and produce soluble proteins that are otherwise difficult to obtain using conventional methods. ...[more]
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