Definition of a bacterial type IV secretion pathway for a DNA substrate.
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ABSTRACT:
SUBMITTER: Cascales E
PROVIDER: S-EPMC3882297 | biostudies-literature | 2004 May
REPOSITORIES: biostudies-literature
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Definition of a bacterial type IV secretion pathway for a DNA substrate.
Cascales Eric E Christie Peter J PJ
Science (New York, N.Y.) 20040501 5674
Bacteria use conjugation systems, a subfamily of the type IV secretion systems, to transfer DNA to recipient cells. Despite 50 years of research, the architecture and mechanism of action of the channel mediating DNA transfer across the bacterial cell envelope remains obscure. By use of a sensitive, quantifiable assay termed transfer DNA immunoprecipitation (TrIP), we identify contacts between a DNA substrate (T-DNA) and 6 of 12 components of the VirB/D4 conjugation system of the phytopathogen Ag ...[more]
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