Ontology highlight
ABSTRACT:
SUBMITTER: Xue J
PROVIDER: S-EPMC3882362 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Xue Jing J Zhou Jie J Zempleni Janos J
American journal of physiology. Cell physiology 20131016 12
In a recent mass spectrometry screen, we identified 108 new proteins that were modified endogenously by covalent binding of biotin; members of the heat shock superfamily of proteins, including heat shock protein 72 (HSP72), were overrepresented among the biotinylated proteins. Mammals respond to infections by secreting extracellular HSP72 (eHSP72), which elicits an immune response. Here, using mass spectrometry and site-directed mutagenesis, we identified five biotinylation sites in HSP72. We us ...[more]