Hepatitis B virus subgenotype A1 predominates in liver disease patients from Kerala, India.

ABSTRACT:

Aim

To molecularly characterize hepatitis B virus (HBV) isolates from Kerala and to relate them to the clinical manifestation of infection.

Methods

Sera and clinical data were collected from 91 patients diagnosed with chronic HBV infection and HBV-related hepatocellular carcinoma (HCC). HBV from 44 HCC, 22 cirrhotic and 25 chronic hepatitis patients were genotyped by sequencing of the complete S region or by restriction fragment length polymorphism assays. The basic core promoter/precore region was sequenced. The complete surface DNA sequences were assembled and aligned manually, and then compared with the sequences of HBV of genotypes (A-J) from GenBank. The evolutionary history was inferred using the Neighbor-Joining method and the evolutionary distances computed using the

SUBMITTER: Gopalakrishnan D 

PROVIDER: S-EPMC3882402 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature