Two barriers or not? Dynamic force spectroscopy on the integrin ?7?1 invasin complex.
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ABSTRACT:
SUBMITTER: Boye K
PROVIDER: S-EPMC3882471 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Publications
Two barriers or not? Dynamic force spectroscopy on the integrin α7β1 invasin complex.
Boye Kristian K Ligezowska Agnieszka A Eble Johannes A JA Hoffmann Bernd B Klösgen Beate B Merkel Rudolf R
Biophysical journal 20131201 12
Dynamic force spectroscopy was used to test force-induced dissociation of the complex between the integrin α7β1 and the bacterial protein invasin. Both proteins were used in truncated forms comprising the respective binding sites. Using the biomembrane force-probe, the bond system was exposed to 14 different loading rates ranging from 18 pN/s to 5.3 nN/s. At each rate, bond rupture spectra were collected. Median forces ranged from 8 to 72 pN. These showed two linear regimes when plotted against ...[more]
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