The architecture of yeast DNA polymerase ?.

ABSTRACT: DNA polymerase ? (Pol?) is specialized for the extension step of translesion DNA synthesis (TLS). Despite its central role in maintaining genome integrity, little is known about its overall architecture. Initially identified as a heterodimer of the catalytic subunit Rev3 and the accessory subunit Rev7, yeast Pol? has recently been shown to form a stable four-subunit enzyme (Pol?-d) upon the incorporation of Pol31 and Pol32, the accessory subunits of yeast Pol?. To understand the 3D architecture and assembly of Pol? and Pol?-d, we employed electron microscopy. We show here how the catalytic and accessory subunits of Pol? and Pol?-d are organized relative to each other. In particular, we show that Pol?-d has a bilobal architecture resembling the replicative polymerases and that Pol32 lies in proximity to Rev7. Collectively, our study provides views of Pol? and Pol?-d and a structural framework for understanding their roles in DNA damage bypass.

SUBMITTER: Gomez-Llorente Y 

PROVIDER: S-EPMC3883112 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature