Dataset Information

Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation.

ABSTRACT: Olfactory receptors (ORs) are expressed in the olfactory epithelium, where they detect odorants, but also in other tissues with additional functions. Some ORs are even overexpressed in tumor cells. In this study, we identified ORs expressed in enterochromaffin tumor cells by RT-PCR, showing that single cells can co-express several ORs. Some of the receptors identified were already reported in other tumors, but they are orphan (without known ligand), as it is the case for most of the hundreds of human ORs. Thus, genes coding for human ORs with known ligands were transfected into these cells, expressing functional heterologous ORs. The in vitro stimulation of these cells by the corresponding OR odorant agonists promoted cell invasion of collagen gels. Using LNCaP prostate cancer cells, the stimulation of the PSGR (Prostate Specific G protein-coupled Receptor), an endogenously overexpressed OR, by ?-ionone, its odorant agonist, resulted in the same phenotypic change. We also showed the involvement of a PI3 kinase ? dependent signaling pathway in this promotion of tumor cell invasiveness triggered by OR stimulation. Finally, after subcutaneous inoculation of LNCaP cells into NSG immunodeficient mice, the in vivo stimulation of these cells by the PSGR agonist ?-ionone significantly enhanced metastasis emergence and spreading.

SUBMITTER: Sanz G

PROVIDER: S-EPMC3885679 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation.

Sanz Guenhaël G Leray Isabelle I Dewaele Aurélie A Sobilo Julien J Lerondel Stéphanie S Bouet Stéphan S Grébert Denise D Monnerie Régine R Pajot-Augy Edith E Mir Lluis M LM

PloS one 20140108 1

Olfactory receptors (ORs) are expressed in the olfactory epithelium, where they detect odorants, but also in other tissues with additional functions. Some ORs are even overexpressed in tumor cells. In this study, we identified ORs expressed in enterochromaffin tumor cells by RT-PCR, showing that single cells can co-express several ORs. Some of the receptors identified were already reported in other tumors, but they are orphan (without known ligand), as it is the case for most of the hundreds of ...[more]

PMID: 24416348

Similar Datasets

Project description:Core fucosylation (addition of fucose in ?-1,6-linkage to core N-acetylglucosamine of N-glycans) catalyzed by fucosyltransferase 8 (FUT8) is critical for signaling receptors involved in many physiological and pathological processes such as cell growth, adhesion, and tumor metastasis. Transforming growth factor-? (TGF-?)-induced epithelial-mesenchymal transition (EMT) regulates the invasion and metastasis of breast tumors. However, whether receptor core fucosylation affects TGF-? signaling during breast cancer progression remains largely unknown.In this study, gene expression profiling and western blot were used to validate the EMT-associated expression of FUT8. Lentivirus-mediated gain-of-function study, short hairpin RNA (shRNA) or CRISPR/Cas9-mediated loss-of-function studies and pharmacological inhibition of FUT8 were used to elucidate the molecular function of FUT8 during TGF-?-induced EMT in breast carcinoma cells. In addition, lectin blot, luciferase assay, and in vitro ligand binding assay were employed to demonstrate the involvement of FUT8 in the TGF-?1 signaling pathway. The role of FUT8 in breast cancer migration, invasion, and metastasis was confirmed using an in vitro transwell assay and mammary fat pad xenograft in vivo tumor model.Gene expression profiling analysis revealed that FUT8 is upregulated in TGF-?-induced EMT; the process was associated with the migratory and invasive abilities of several breast carcinoma cell lines. Gain-of-function and loss-of-function studies demonstrated that FUT8 overexpression stimulated the EMT process, whereas FUT8 knockdown suppressed the invasiveness of highly aggressive breast carcinoma cells. Furthermore, TGF-? receptor complexes might be core fucosylated by FUT8 to facilitate TGF-? binding and enhance downstream signaling. Importantly, FUT8 inhibition suppressed the invasive ability of highly metastatic breast cancer cells and impaired their lung metastasis.Our results reveal a positive feedback mechanism of FUT8-mediated receptor core fucosylation that promotes TGF-? signaling and EMT, thus stimulating breast cancer cell invasion and metastasis.

Dataset Information

Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation.

Publications

Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure