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Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis.

ABSTRACT: Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.

SUBMITTER: Garcia A

PROVIDER: S-EPMC3885745 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis.

García Alfredo A Martínez Remigio R Benitez-Medina José Manuel JM Risco David D Garcia Waldo Luis WL Rey Joaquín J Alonso Juan Manuel JM Hermoso de Mendoza Javier J

Journal of veterinary science 20130630 4

Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was use ...[more]

PMID: 23820221

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