Lipid directed intrinsic membrane protein segregation.

ABSTRACT: We demonstrate a new approach for direct reconstitution of membrane proteins during giant vesicle formation. We show that it is straightforward to create a tissue-like giant vesicle film swelled with membrane protein using aquaporin SoPIP2;1 as an illustration. These vesicles can also be easily harvested for individual study. By controlling the lipid composition we are able to direct the aquaporin into specific immiscible liquid domains in giant vesicles. The oligomeric ?-helical protein cosegregates with the cholesterol-poor domains in phase separating ternary mixtures.

SUBMITTER: Hansen JS 

PROVIDER: S-EPMC3886709 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature