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Lipid directed intrinsic membrane protein segregation.

ABSTRACT: We demonstrate a new approach for direct reconstitution of membrane proteins during giant vesicle formation. We show that it is straightforward to create a tissue-like giant vesicle film swelled with membrane protein using aquaporin SoPIP2;1 as an illustration. These vesicles can also be easily harvested for individual study. By controlling the lipid composition we are able to direct the aquaporin into specific immiscible liquid domains in giant vesicles. The oligomeric ?-helical protein cosegregates with the cholesterol-poor domains in phase separating ternary mixtures.

SUBMITTER: Hansen JS

PROVIDER: S-EPMC3886709 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Lipid directed intrinsic membrane protein segregation.

Hansen Jesper S JS Thompson James R JR Hélix-Nielsen Claus C Malmstadt Noah N

Journal of the American Chemical Society 20131111 46

We demonstrate a new approach for direct reconstitution of membrane proteins during giant vesicle formation. We show that it is straightforward to create a tissue-like giant vesicle film swelled with membrane protein using aquaporin SoPIP2;1 as an illustration. These vesicles can also be easily harvested for individual study. By controlling the lipid composition we are able to direct the aquaporin into specific immiscible liquid domains in giant vesicles. The oligomeric α-helical protein cosegre ...[more]

PMID: 24180248

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