Orosphere assay: a method for propagation of head and neck cancer stem cells.
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ABSTRACT:
SUBMITTER: Krishnamurthy S
PROVIDER: S-EPMC3887391 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Publications
Orosphere assay: a method for propagation of head and neck cancer stem cells.
Krishnamurthy Sudha S Nör Jacques E JE
Head & neck 20120713 7
<h4>Background</h4>Recent evidence suggests that head and neck squamous cell carcinomas (HNSCCs) harbor a small subpopulation of highly tumorigenic cells, designated cancer stem cells. A limiting factor in cancer stem cell research is the intrinsic difficulty of expanding cells in an undifferentiated state in vitro.<h4>Methods</h4>Here, we describe the development of the orosphere assay, a method for the study of putative head and neck cancer stem cells. An orosphere is defined as a nonadherent ...[more]
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