Chemical suppression of defects in mitotic spindle assembly, redox control, and sterol biosynthesis by hydroxyurea.

ABSTRACT: We describe the results of a systematic search for a class of hitherto-overlooked chemical-genetic interactions in the Saccharomyces cerevisiae genome, which exists between a detrimental genetic mutation and a chemical/drug that can ameliorate, rather than exacerbate, that detriment. We refer to this type of interaction as "chemical suppression." Our work was driven by the hypothesis that genome instability in a certain class of mutants could be alleviated by mild replication inhibition using chemicals/drugs. We queried a collection of conditionally lethal, i.e., temperature-sensitive, alleles representing 40% of the yeast essential genes for those mutants whose growth defect can be suppressed by hydroxyurea (HU), known as a potent DNA replication inhibitor, at the restrictive temperature.

SUBMITTER: McCulley A 

PROVIDER: S-EPMC3887538 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature