A mutational study of Cnu reveals attractive forces between Cnu and H-NS.
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ABSTRACT:
SUBMITTER: Yun SH
PROVIDER: S-EPMC3887714 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Publications
A mutational study of Cnu reveals attractive forces between Cnu and H-NS.
Yun Sang Hoon SH Ji Sang Chun SC Jeon Heung Jin HJ Wang Xun X Lee Younghoon Y Choi Byong-Seok BS Lim Heon M HM
Molecules and cells 20120215 2
Cnu is a small 71-amino acid protein that complexes with H-NS and binds to a specific sequence in the replication origin of the E. coli chromosome. To understand the mechanism of interaction between Cnu and H-NS, we used bacterial genetics to select and analyze Cnu variants that cannot complex with H-NS. Out of 2,000 colonies, 40 Cnu variants were identified. Most variants (82.5%) had a single mutation, but a few variants (17.5%) had double amino acid changes. An in vitro assay was used to ident ...[more]
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