Unknown

Dataset Information

0

Characterization of cell death induced by NbBPS1 silencing in Nicotiana benthamiana.

ABSTRACT: We previously showed that silencing of NbBPS1 encoding an endoplasmic reticulum (ER)-localized protein results in pleiotrophic developmental defects and cell death in Nicotiana benthamiana [Kang et al. (2008)]. In this study, we investigated the mechanism of the cell death caused by NbBPS1 silencing. Affected leaf cells exhibited morphological markers of programmed cell death (PCD) and accumulated excessive amounts of reactive oxygen species. NbBPS1 silencing caused dramatic induction of the ER stress marker genes BiP-like protein (BLP) genes, HSP70, and Bax Inhibitor-1. Furthermore, NbBPS1 deficiency led to relocalization of bZIP28 transcription factor from the ER membrane to the nucleus, similar to the bZIP28 relocalization during tunicamycin-induced ER stress. Abnormal accumulation of vesicles and increased autophagy activity were also observed in the affected leaf cells. These results suggest that inactivation of NbBPS1 function in the ER leads to ER stress, autophagy, and PCD activation in N. benthamiana.

SUBMITTER: Kang YW 

PROVIDER: S-EPMC3887818 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Characterization of cell death induced by NbBPS1 silencing in Nicotiana benthamiana.

Kang Yong Won YW   Jeon Young Y   Pai Hyun-Sook HS  

Molecules and cells 20120622 2

We previously showed that silencing of NbBPS1 encoding an endoplasmic reticulum (ER)-localized protein results in pleiotrophic developmental defects and cell death in Nicotiana benthamiana [Kang et al. (2008)]. In this study, we investigated the mechanism of the cell death caused by NbBPS1 silencing. Affected leaf cells exhibited morphological markers of programmed cell death (PCD) and accumulated excessive amounts of reactive oxygen species. NbBPS1 silencing caused dramatic induction of the ER  ...[more]

Similar Datasets

Unknown Silencing of Nicotiana benthamiana Neuroblastoma-Amplified Gene causes ER stress and cell death.
| S-EPMC3654999 | biostudies-literature
Unknown Silencing of phosphoinositide dependent protein kinase orthologs reduces hypersensitive cell death in <i>Nicotiana benthamiana</i>.
| S-EPMC7557664 | biostudies-literature
Unknown Sugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana.
| S-EPMC5903913 | biostudies-literature
Unknown Jasmonate ZIM-domain (JAZ) protein regulates host and nonhost pathogen-induced cell death in tomato and Nicotiana benthamiana.
| S-EPMC3785428 | biostudies-literature
Unknown Transient Expression of Candidatus Liberibacter Asiaticus Effector Induces Cell Death in Nicotiana benthamiana.
| S-EPMC4933711 | biostudies-literature
Unknown Molecular Characterization of A Novel Effector Expansin-like Protein from Heterodera avenae that Induces Cell Death in Nicotiana benthamiana.
| S-EPMC5093861 | biostudies-literature
Unknown An efficient Potato virus X -based microRNA silencing in Nicotiana benthamiana.
| S-EPMC4738334 | biostudies-literature
Unknown An Asparagine-Rich Protein Nbnrp1 Modulate Verticillium dahliae Protein PevD1-Induced Cell Death and Disease Resistance in Nicotiana benthamiana.
| S-EPMC5846053 | biostudies-literature
Unknown NbALY916 is involved in potato virus X P25-triggered cell death in Nicotiana benthamiana.
| S-EPMC7549001 | biostudies-literature
Unknown Involvement of RDR6 in short-range intercellular RNA silencing in Nicotiana benthamiana.
| S-EPMC3381291 | biostudies-literature