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Experimentally induced blood-stage Plasmodium vivax infection in healthy volunteers.


ABSTRACT: Major impediments to development of vaccines and drugs for Plasmodium vivax malaria are the inability to culture this species and the extreme difficulty in undertaking clinical research by experimental infection.A parasite bank was collected from a 49-year-old woman with P. vivax infection, characterized, and used in an experimental infection study.The donor made a full recovery from malaria after collection of a parasite bank, which tested negative for agents screened for in blood donations. DNA sequence analysis of the isolate indicated that it was clonal. Two subjects inoculated with the isolate became polymerase chain reaction positive on days 8 and 9, with onset of symptoms and positive blood smears on day 14, when they were treated with artemether-lumefantrine, with rapid clinical and parasitologic response. Transcripts of the parasite gene pvs25 that is expressed in gametocytes, the life cycle stage infectious to mosquitoes, were first detected on days 11 and 12.This experimental system results in in vivo parasite growth, probably infectious to mosquitoes. It offers the opportunity to undertake studies previously impossible in P. vivax that will facilitate a better understanding of the pathology of vivax malaria and development of antimalarial drugs and vaccines. Trial Registration. ANZCTR: 12612001096842.

SUBMITTER: McCarthy JS 

PROVIDER: S-EPMC3888148 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Experimentally induced blood-stage Plasmodium vivax infection in healthy volunteers.

McCarthy James S JS   Griffin Paul M PM   Sekuloski Silvana S   Bright A Taylor AT   Rockett Rebecca R   Looke David D   Elliott Suzanne S   Whiley David D   Sloots Theo T   Winzeler Elizabeth A EA   Trenholme Katharine R KR  

The Journal of infectious diseases 20130801 10


<h4>Background</h4>Major impediments to development of vaccines and drugs for Plasmodium vivax malaria are the inability to culture this species and the extreme difficulty in undertaking clinical research by experimental infection.<h4>Methods</h4>A parasite bank was collected from a 49-year-old woman with P. vivax infection, characterized, and used in an experimental infection study.<h4>Results</h4>The donor made a full recovery from malaria after collection of a parasite bank, which tested nega  ...[more]

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