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Modification of clearview tuberculosis (TB) enzyme-linked immunosorbent assay for TB patients not infected with HIV.


ABSTRACT: Diagnosis of active tuberculosis by detection of urinary lipoarabinomannan (uLAM) from Mycobacterium tuberculosis is an attractive approach. Concentrating urine 100-fold allowed quantitation of uLAM at levels equal to picograms/ml of nonconcentrated urine. The approach of concentrating urine 100-fold improved the clinical sensitivity of the Clearview TB enzyme-linked immunosorbent assay (ELISA) from 7% to 57% yet impaired its specificity from 97% to 89%. (This study has been registered at University Hospital of Turku under registration no. 47/180/2009, Helsinki University Central Hospital under 149/2010, University Hospital of Kuopio under 105/2010, and China Medical University Hospital, Taichung, under DMR-99-IRB-075-2.).

SUBMITTER: Savolainen L 

PROVIDER: S-EPMC3889579 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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Modification of clearview tuberculosis (TB) enzyme-linked immunosorbent assay for TB patients not infected with HIV.

Savolainen Laura L   Kantele Anu A   Sandboge Bettina B   Sirén Marita M   Valleala Heikki H   Tuompo Riitta R   Pusa Liana L   Erkinjuntti-Pekkanen Riitta R   Knuuttila Aija A   Ku Cheng-Lung CL   Chi Chih-Yu CY   Vasankari Tuula T   Tuuminen Tamara T  

Clinical and vaccine immunology : CVI 20130703 9


Diagnosis of active tuberculosis by detection of urinary lipoarabinomannan (uLAM) from Mycobacterium tuberculosis is an attractive approach. Concentrating urine 100-fold allowed quantitation of uLAM at levels equal to picograms/ml of nonconcentrated urine. The approach of concentrating urine 100-fold improved the clinical sensitivity of the Clearview TB enzyme-linked immunosorbent assay (ELISA) from 7% to 57% yet impaired its specificity from 97% to 89%. (This study has been registered at Univer  ...[more]

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