Ontology highlight
ABSTRACT:
SUBMITTER: Bhargava V
PROVIDER: S-EPMC3890974 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Bhargava Vipul V Head Steven R SR Ordoukhanian Phillip P Mercola Mark M Subramaniam Shankar S
Scientific reports 20140114
Recent advances in RNA-seq methodologies from limiting amounts of mRNA have facilitated the characterization of rare cell-types in various biological systems. So far, however, technical variations in these methods have not been adequately characterized, vis-à-vis sensitivity, starting with reduced levels of mRNA. Here, we generated sequencing libraries from limiting amounts of mRNA using three amplification-based methods, viz. Smart-seq, DP-seq and CEL-seq, and demonstrated significant technical ...[more]