Ontology highlight
ABSTRACT:
SUBMITTER: Anagnostou VK
PROVIDER: S-EPMC3891912 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Anagnostou Valsamo K VK Welsh Allison W AW Giltnane Jennifer M JM Siddiqui Summar S Liceaga Camil C Gustavson Mark M Syrigos Konstantinos N KN Reiter Jill L JL Rimm David L DL
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 20100323 4
<h4>Background</h4>Despite the widespread use of immunohistochemistry (IHC), there are no standardization guidelines that control for antibody probe variability. Here we describe the effect of variable antibody reagents in the assessment of cancer-related biomarkers by IHC.<h4>Methods</h4>Estrogen receptor (ER), epidermal growth factor receptor (EGFR) 1, and human epidermal growth factor receptor 3 (HER3) were evaluated by quantitative immunofluorescence. Correlations between ER clones 1D5, SP1, ...[more]