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Targeting cancer cells with reactive oxygen and nitrogen species generated by atmospheric-pressure air plasma.


ABSTRACT: The plasma jet has been proposed as a novel therapeutic method for cancer. Anticancer activity of plasma has been reported to involve mitochondrial dysfunction. However, what constituents generated by plasma is linked to this anticancer process and its mechanism of action remain unclear. Here, we report that the therapeutic effects of air plasma result from generation of reactive oxygen/nitrogen species (ROS/RNS) including H2O2, Ox, OH-, •O2, NOx, leading to depolarization of mitochondrial membrane potential and mitochondrial ROS accumulation. Simultaneously, ROS/RNS activate c-Jun NH2-terminal kinase (JNK) and p38 kinase. As a consequence, treatment with air plasma jets induces apoptotic death in human cervical cancer HeLa cells. Pretreatment of the cells with antioxidants, JNK and p38 inhibitors, or JNK and p38 siRNA abrogates the depolarization of mitochondrial membrane potential and impairs the air plasma-induced apoptotic cell death, suggesting that the ROS/RNS generated by plasma trigger signaling pathways involving JNK and p38 and promote mitochondrial perturbation, leading to apoptosis. Therefore, administration of air plasma may be a feasible strategy to eliminate cancer cells.

SUBMITTER: Ahn HJ 

PROVIDER: S-EPMC3897664 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Targeting cancer cells with reactive oxygen and nitrogen species generated by atmospheric-pressure air plasma.

Ahn Hak Jun HJ   Kim Kang Il KI   Hoan Nguyen Ngoc NN   Kim Churl Ho CH   Moon Eunpyo E   Choi Kyeong Sook KS   Yang Sang Sik SS   Lee Jong-Soo JS  

PloS one 20140121 1


The plasma jet has been proposed as a novel therapeutic method for cancer. Anticancer activity of plasma has been reported to involve mitochondrial dysfunction. However, what constituents generated by plasma is linked to this anticancer process and its mechanism of action remain unclear. Here, we report that the therapeutic effects of air plasma result from generation of reactive oxygen/nitrogen species (ROS/RNS) including H2O2, Ox, OH-, •O2, NOx, leading to depolarization of mitochondrial membr  ...[more]

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