Nanopore-based identification of individual nucleotides for direct RNA sequencing.
Ontology highlight
ABSTRACT:
SUBMITTER: Ayub M
PROVIDER: S-EPMC3899427 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Nanopore-based identification of individual nucleotides for direct RNA sequencing.
Ayub Mariam M Hardwick Steven W SW Luisi Ben F BF Bayley Hagan H
Nano letters 20131113 12
We describe a label-free ribobase identification method, which uses ionic current measurement to resolve ribonucleoside monophosphates or diphosphates in α-hemolysin protein nanopores containing amino-cyclodextrin adapters. The accuracy of base identification is further investigated through the use of a guanidino-modified adapter. On the basis of these findings, an exosequencing approach is envisioned in which a processive exoribonuclease (polynucleotide phosphorylase) presents sequentially clea ...[more]
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