Ontology highlight
ABSTRACT:
SUBMITTER: Ferrie AM
PROVIDER: S-EPMC3899747 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Ferrie Ann M AM Sun Haiyan H Zaytseva Natalya N Fang Ye Y
Scientific reports 20140123
We present subclone sensitive cell phenotypic pharmacology of ligands at the β2-adrenergic receptor (β2-AR) stably expressed in HEK-293 cells. The parental cell line was transfected with green fluorescent protein (GFP)-tagged β2-AR. Four stable subclones were established and used to profile a library of sixty-nine AR ligands. Dynamic mass redistribution (DMR) profiling resulted in a pharmacological activity map suggesting that HEK293 endogenously expresses functional Gi-coupled α2-AR and Gs-coup ...[more]