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Single-molecule analysis of gene expression using two-color RNA labeling in live yeast.

ABSTRACT: Live-cell imaging of mRNA yields important insights into gene expression, but it has generally been limited to the labeling of one RNA species and has never been used to count single mRNAs over time in yeast. We demonstrate a two-color imaging system with single-molecule resolution using MS2 and PP7 RNA labeling. We use this methodology to measure intrinsic noise in mRNA levels and RNA polymerase II kinetics at a single gene.

SUBMITTER: Hocine S

PROVIDER: S-EPMC3899799 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Single-molecule analysis of gene expression using two-color RNA labeling in live yeast.

Hocine Sami S Raymond Pascal P Zenklusen Daniel D Chao Jeffrey A JA Singer Robert H RH

Nature methods 20121223 2

Live-cell imaging of mRNA yields important insights into gene expression, but it has generally been limited to the labeling of one RNA species and has never been used to count single mRNAs over time in yeast. We demonstrate a two-color imaging system with single-molecule resolution using MS2 and PP7 RNA labeling. We use this methodology to measure intrinsic noise in mRNA levels and RNA polymerase II kinetics at a single gene. ...[more]

PMID: 23263691

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