Ontology highlight
ABSTRACT:
SUBMITTER: DiMaio F
PROVIDER: S-EPMC3899897 | biostudies-literature | 2009 Sep
REPOSITORIES: biostudies-literature
DiMaio Frank F Tyka Michael D MD Baker Matthew L ML Chiu Wah W Baker David D
Journal of molecular biology 20090708 1
We describe a method based on Rosetta structure refinement for generating high-resolution, all-atom protein models from electron cryomicroscopy density maps. A local measure of the fit of a model to the density is used to directly guide structure refinement and to identify regions incompatible with the density that are then targeted for extensive rebuilding. Over a range of test cases using both simulated and experimentally generated data, the method consistently increases the accuracy of starti ...[more]