Ontology highlight
ABSTRACT:
SUBMITTER: Lijkwan MA
PROVIDER: S-EPMC3900020 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Human gene therapy 20140107 1
In this study, we target the hypoxia inducible factor-1 alpha (HIF-1-alpha) pathway by short hairpin RNA interference therapy targeting prolyl hydroxylase-2 (shPHD2). We use the minicircle (MC) vector technology as an alternative for conventional nonviral plasmid (PL) vectors in order to improve neovascularization after unilateral hindlimb ischemia in a murine model. Gene expression and transfection efficiency of MC and PL, both in vitro and in vivo, were assessed using bioluminescence imaging ( ...[more]