Ontology highlight
ABSTRACT:
SUBMITTER: Foulds CE
PROVIDER: S-EPMC3900250 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Foulds Charles E CE Feng Qin Q Ding Chen C Bailey Suzanna S Hunsaker Tamra L TL Malovannaya Anna A Hamilton Ross A RA Gates Leah A LA Zhang Zheng Z Li Chunshu C Chan Doug D Bajaj Amol A Callaway Celetta G CG Edwards Dean P DP Lonard David M DM Tsai Sophia Y SY Tsai Ming-Jer MJ Qin Jun J O'Malley Bert W BW
Molecular cell 20130711 2
Chromatin immunoprecipitation studies have mapped protein occupancies at many genomic loci. However, a detailed picture of the complexity of coregulators (CoRs) bound to a defined enhancer along with a transcription factor is missing. To address this, we used biotin-DNA pull-down assays coupled with mass spectrometry-immunoblotting to identify at least 17 CoRs from nuclear extracts bound to 17β-estradiol (E2)-liganded estrogen receptor-α on estrogen response elements (EREs). Unexpectedly, these ...[more]