Ontology highlight
ABSTRACT:
SUBMITTER: Tsvetkov AS
PROVIDER: S-EPMC3900497 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Tsvetkov Andrey S AS Arrasate Montserrat M Barmada Sami S Ando D Michael DM Sharma Punita P Shaby Benjamin A BA Finkbeiner Steven S
Nature chemical biology 20130721 9
In polyglutamine (polyQ) diseases, only certain neurons die, despite widespread expression of the offending protein. PolyQ expansion may induce neurodegeneration by impairing proteostasis, but protein aggregation and toxicity tend to confound conventional measurements of protein stability. Here, we used optical pulse labeling to measure effects of polyQ expansions on the mean lifetime of a fragment of huntingtin, the protein that causes Huntington's disease, in living neurons. We show that polyQ ...[more]