Ontology highlight
ABSTRACT:
SUBMITTER: Kudla G
PROVIDER: S-EPMC3902468 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Kudla Grzegorz G Murray Andrew W AW Tollervey David D Plotkin Joshua B JB
Science (New York, N.Y.) 20090401 5924
Synonymous mutations do not alter the encoded protein, but they can influence gene expression. To investigate how, we engineered a synthetic library of 154 genes that varied randomly at synonymous sites, but all encoded the same green fluorescent protein (GFP). When expressed in Escherichia coli, GFP protein levels varied 250-fold across the library. GFP messenger RNA (mRNA) levels, mRNA degradation patterns, and bacterial growth rates also varied, but codon bias did not correlate with gene expr ...[more]