Survival of neural stem cells undergoing DNA damage-induced astrocytic differentiation in self-renewal-promoting conditions in vitro.

ABSTRACT: We recently reported that neural stem cells (NSCs) become senescent and commit to astrocytic differentiation upon X-ray irradiation. Surprisingly, under self-renewing culture conditions, some of these senescent cells undergo p53-independent apoptosis, which can be suppressed by caspase inhibition and BCL2 overexpression. Inhibition of apoptosis proved beneficial for astroglial differentiation efficiency; hence the toxicity of DNA damage on NSCs was specifically tested in context of the culture conditions. In this regard, self-renewal-promoting culture conditions proved incompatible with terminal astrocyte differentiation and impacted negatively on the viability of NSCs following DNA damage-induced cell cycle exit. On the contrary, a switch to differentiation-supporting conditions ablated a

SUBMITTER: Schneider L 

PROVIDER: S-EPMC3903639 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature