Ontology highlight
ABSTRACT:
SUBMITTER: Taylor MS
PROVIDER: S-EPMC3904357 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Taylor Martin S MS Taylor Martin S MS LaCava John J Mita Paolo P Molloy Kelly R KR Huang Cheng Ran Lisa CR Li Donghui D Adney Emily M EM Jiang Hua H Burns Kathleen H KH Chait Brian T BT Rout Michael P MP Boeke Jef D JD Dai Lixin L
Cell 20131101 5
LINE-1s are active human DNA parasites that are agents of genome dynamics in evolution and disease. These streamlined elements require host factors to complete their life cycles, whereas hosts have developed mechanisms to combat retrotransposition's mutagenic effects. As such, endogenous L1 expression levels are extremely low, creating a roadblock for detailed interactomic analyses. Here, we describe a system to express and purify highly active L1 RNP complexes from human suspension cell culture ...[more]