Ontology highlight
ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC3904521 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Liu Yuwen Y Zhou Jie J White Kevin P KP
Bioinformatics (Oxford, England) 20131206 3
<h4>Motivation</h4>RNA-seq is replacing microarrays as the primary tool for gene expression studies. Many RNA-seq studies have used insufficient biological replicates, resulting in low statistical power and inefficient use of sequencing resources.<h4>Results</h4>We show the explicit trade-off between more biological replicates and deeper sequencing in increasing power to detect differentially expressed (DE) genes. In the human cell line MCF7, adding more sequencing depth after 10 M reads gives d ...[more]