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Association of insulin-like growth factor 2 with the insulin-linked polymorphic region in cultured fetal thymus cells.

ABSTRACT: The insulin-linked polymorphic region (ILPR) is a regulatory sequence in the promoter region upstream of the human insulin gene and is widely recognized as a locus of type 1 diabetes susceptibility. Polymorphism of the ILPR sequence can affect expression of both insulin and the adjacent insulin-like growth factor 2 (IGF-2) gene. Several ILPR variants form G-quadruplex DNA structures in vitro that exhibit affinity binding to insulin and IGF-2. It has been suggested that the ILPR may form G-quadruplexes in vivo as well, raising the possibility that insulin and IGF-2 may bind to these structures in the ILPR in chromatin of live cells. This work establishes the presence of IGF-2 in the nucleus of cells cultured from human fetal thymus and its association with the ILPR in the chromatin of these cells. In vitro experiments support the involvement of G-quadruplex DNA in the binding interaction.

SUBMITTER: Wang Y

PROVIDER: S-EPMC3906629 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature

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Association of insulin-like growth factor 2 with the insulin-linked polymorphic region in cultured fetal thymus cells.

Wang Yuexi Y Zhang Huiping H Ligon Lee A LA McGown Linda B LB

Biochemistry 20090901 34

The insulin-linked polymorphic region (ILPR) is a regulatory sequence in the promoter region upstream of the human insulin gene and is widely recognized as a locus of type 1 diabetes susceptibility. Polymorphism of the ILPR sequence can affect expression of both insulin and the adjacent insulin-like growth factor 2 (IGF-2) gene. Several ILPR variants form G-quadruplex DNA structures in vitro that exhibit affinity binding to insulin and IGF-2. It has been suggested that the ILPR may form G-quadru ...[more]

PMID: 19588890

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