Dynamics of the serine chemoreceptor in the Escherichia coli inner membrane: a high-speed single-molecule tracking study.
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ABSTRACT:
SUBMITTER: Oh D
PROVIDER: S-EPMC3907255 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
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Dynamics of the serine chemoreceptor in the Escherichia coli inner membrane: a high-speed single-molecule tracking study.
Oh Dongmyung D Yu Yang Y Lee Hochan H Wanner Barry L BL Ritchie Ken K
Biophysical journal 20140101 1
We investigated the mobility of the polar localized serine chemoreceptor, Tsr, labeled by the fluorescent protein Venus in the inner membrane of live Escherichia coli cells at observation rates up to 1000 Hz. A fraction (7%) of all Tsr molecules shows free diffusion over the entire cell surface with an average diffusion coefficient of 0.40 ± 0.01 μm(2) s(-1). The remaining molecules were found to be ultimately confined in compartments of size 290 ± 15 nm and showed restricted diffusion at an inn ...[more]
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