Ontology highlight
ABSTRACT:
SUBMITTER: Vishnu N
PROVIDER: S-EPMC3907277 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Vishnu Neelanjan N Jadoon Khan Muhammad M Karsten Felix F Groschner Lukas N LN Waldeck-Weiermair Markus M Rost Rene R Hallström Seth S Imamura Hiromi H Graier Wolfgang F WF Malli Roland R
Molecular biology of the cell 20131204 3
Multiple functions of the endoplasmic reticulum (ER) essentially depend on ATP within this organelle. However, little is known about ER ATP dynamics and the regulation of ER ATP import. Here we describe real-time recordings of ER ATP fluxes in single cells using an ER-targeted, genetically encoded ATP sensor. In vitro experiments prove that the ATP sensor is both Ca(2+) and redox insensitive, which makes it possible to monitor Ca(2+)-coupled ER ATP dynamics specifically. The approach uncovers a ...[more]