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Wheat V-H+-ATPase subunit genes significantly affect salt tolerance in Arabidopsis thaliana.


ABSTRACT: Genes for V-H(+)-ATPase subunits were identified and cloned from the salt-tolerant wheat mutant RH8706-49. Sequences of these genes are highly conserved in plants. Overexpression of these genes in Arabidopsis thaliana improved its salt tolerance, and increased the activities of V-H(+)-ATPase and Na(+)/H(+) exchange, with the largest increase in plants carrying the c subunit of V-H(+)-ATPase. Results from quantitative RT-PCR analysis indicated that the mRNA level of each V-H(+)-ATPase subunit in the Arabidopsis increased under salt stress. Overall, our results suggest that each V-H(+)-ATPase subunit plays a key role in enhancing salt tolerance in plants.

SUBMITTER: He X 

PROVIDER: S-EPMC3907383 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Wheat V-H+-ATPase subunit genes significantly affect salt tolerance in Arabidopsis thaliana.

He Xiaoliang X   Huang Xi X   Shen Yinzhu Y   Huang Zhanjing Z  

PloS one 20140130 1


Genes for V-H(+)-ATPase subunits were identified and cloned from the salt-tolerant wheat mutant RH8706-49. Sequences of these genes are highly conserved in plants. Overexpression of these genes in Arabidopsis thaliana improved its salt tolerance, and increased the activities of V-H(+)-ATPase and Na(+)/H(+) exchange, with the largest increase in plants carrying the c subunit of V-H(+)-ATPase. Results from quantitative RT-PCR analysis indicated that the mRNA level of each V-H(+)-ATPase subunit in  ...[more]

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