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Isolation of a multi-trait plant growth promoting Brevundimonas sp. and its effect on the growth of Bt-cotton.

ABSTRACT: Arid regions pose a serious problem for crop production by suppressing plant growth. The use of plant growth promoting rhizobacteria (PGPR) as bioinoculant may be promising to enhance the crop yield in arid conditions. In the present investigation, four strains of cultivable bacteria associated with the rhizosphere of Saccharum L. grown in arid region were isolated using N-free media. Assessment of their nitrogen-fixing ability through amplification of nifH gene showed the presence of nifH gene (390 bp) in only one (MDB4) of the four isolates. The nitrogen-fixing potential of this isolate was confirmed by the presence of nitrogenase activity determined using acetylene reduction assay. The diazotrophic MDB4 isolate also exhibited other PGPR traits, such as the production of indole-3-acetic acid (IAA) and ammonia. In pot experiments, inoculation of Bt-cotton seeds with MDB4 enhanced the growth of plants as shown by significant increase in plant height (68.41 %), shoot dry weight (58.44 %) and root dry weight (64.81 %) over untreated control. The MDB4 strain was Gram negative and identified as Brevundimonas sp. on the basis of phenotypic, biochemical, phylogenetic and 16S rRNA gene sequencing data. It is concluded that the MDB4 bacterial strain having different plant growth promoting activities can be considered as a beneficial microbe for sustainable agriculture in arid regions.

SUBMITTER: Kumar V

PROVIDER: S-EPMC3909574 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BackgroundCastor (Ricinus communis L.) is cultivated for seed oil and to feed (leaves) Eri silkworm, Samia ricini (Donovan) Hutt. Alternaria blight affects castor cultivation resulting substantial yield loss (∼30%). Uses of synthetic fertilizers and agrochemicals for disease management have serious concerns as the castor leaves are fed to eri silkworms for rearing. Application of plant growth promoting rhizobacteria for disease suppression and to enhance plant growth will be a healthier choice in castor cultivation. The aim of this study was to assess the efficacy of Alternaria blight disease suppression by native rhizobacteria isolated from wasteland castor and their ability on plant growth promotion.MethodologyWe isolated 50 bacterial antagonists from castor rhizosphere using the dilution plate method and evaluated their antagonistic activity against the castor blight pathogen, Alternaria ricini. Based on antimicrobial bioassay and plant growth promotion (PGP) traits (phosphate solubilization, ACC deaminase activities, production of IAA, GA3, HCN, NH3 and siderophore), salt and acid tolerance; we have chosen ten potential isolates and identified them through 16SrRNA gene sequencing and analysis. Disease suppression and plant growth studies were evaluated in pot experiments.Results and conclusionThree isolates namely, Enterobacter hormaechei (LRP-2), Bacillus mycoides (HF-1) and B. aryabhattai (UR-6) showed potential antagonistic activities and PGP traits which were selected for disease suppression and PGP studies. Application of PGPR consortia (LRP-2+HF-1) could suppress the plants from A. ricini infection in challenged inoculation. Mix inoculation of LRP-2 and UR-6 showed synergistic effect and enhanced plant growth in pot experiments. Combinations of E. hormaechei (LRP-2), B. mycoides (HF-1) and B. aryabhattai (UR-6) can be applied as bio-control and bio-fertilizer formulation to protect castor from Alternaria blight and also to enhance plant growth.

Project description:Drought stress impacts cotton plant growth and productivity across countries. Plants can initiate morphological, cellular, and proteomic changes to adapt to unfavorable conditions. However, our knowledge of how cotton plants respond to drought stress at the proteome level is limited. Herein, we elucidated the molecular coordination underlining the drought tolerance of two inbred cotton varieties, Bacillus thuringiensis-cotton [Bt-cotton + Cry1 Ac gene and Cry 2 Ab gene; NCS BG II BT (BTCS/BTDS)] and Hybrid cotton variety [Non-Bt-cotton; (HCS/HDS)]. Our morphological observations and biochemical experiments showed a different tolerance level between two inbred lines to drought stress. Our proteomic analysis using 2D-DIGE revealed that the changes among them were not obviously in respect to their controls apart from under drought stress, illustrating the differential expression of 509 and 337 proteins in BTDS and HDS compared to their controls. Among these, we identified eight sets of differentially expressed proteins (DEPs) and characterized them using MALDI-TOF/TOF mass spectrometry. Furthermore, the quantitative real-time PCR analysis was carried out with the identified drought-related proteins and confirmed differential expressions. In silico analysis of DEPs using Cytoscape network finds ATPB, NAT9, ERD, LEA, and EMB2001 to be functionally correlative to various drought-responsive genes LEA, AP2/ERF, WRKY, and NAC. These proteins play a vital role in transcriptomic regulation under stress conditions. The higher drought response in Bt cotton (BTCS/BTDS) attributed to the overexpression of photosynthetic proteins enhanced lipid metabolism, increased cellular detoxification and activation chaperones, and reduced synthesis of unwanted proteins. Thus, the Bt variety had enhanced photosynthesis, elevated water retention potential, balanced leaf stomata ultrastructure, and substantially increased antioxidant activity than the Non-Bt cotton. Our results may aid breeders and provide further insights into developing new drought-tolerant and high-yielding cotton hybrid varieties.

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Isolation of a multi-trait plant growth promoting Brevundimonas sp. and its effect on the growth of Bt-cotton.

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