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Comparative Proteomics of Sera From HCC Patients With Different Origins.

ABSTRACT: BACKGROUND:Hepatocellular carcinoma (HCC), a major fatal cancer worldwide, is induced by different etiological factors in the liver. OBJECTIVES:To gain insight into serum protein profiling of HCC with different etiologies. PATIENTS AND METHODS:We subjected the sera of HBV-HCC, HCV-HCC, non-B non-C-HCC patients, and healthy volunteers to two-dimensional gel electrophoresis (2-DE) and liquid chromatography tandem mass spectrometry (LC-MS/MS). RESULTS:We found 30 differentially expressed protein spots (? 1.5 fold P < 0.05) between these two analyses; of them 17 protein spots corresponding to 8 proteins were identified by MS. Transthyretin, leucine rich ?-2-glycoprotein, and ficolin 3 were differentially expressed between HBV-related HCC and non-B non-C-HCC sera. Moreover, haptoglobin ?-2 isoforms were decreased in HCV-HCC compared to non-B non-CHCC. CONCLUSIONS:Serum proteome analyses of HCC with different origins showed a differential protein pattern, presumably related to different hepatopathogenesis in liver induced by different agents. Further studies are required to clarify the importance of identified proteins for early diagnosis of HCC with different origins.

SUBMITTER: Sarvari J

PROVIDER: S-EPMC3909643 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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Comparative Proteomics of Sera From HCC Patients With Different Origins.

Sarvari Jamal J Mojtahedi Zahra Z Kuramitsu Yasuhiro Y Fattahi Mohammad Reza MR Ghaderi Abbas A Nakamura Kazuyuki K Erfani Nasrollah N

Hepatitis monthly 20140119 1

<h4>Background</h4>Hepatocellular carcinoma (HCC), a major fatal cancer worldwide, is induced by different etiological factors in the liver.<h4>Objectives</h4>To gain insight into serum protein profiling of HCC with different etiologies.<h4>Patients and methods</h4>We subjected the sera of HBV-HCC, HCV-HCC, non-B non-C-HCC patients, and healthy volunteers to two-dimensional gel electrophoresis (2-DE) and liquid chromatography tandem mass spectrometry (LC-MS/MS).<h4>Results</h4>We found 30 differ ...[more]

PMID: 24497876

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Project description:Aphids are phloem-feeding insects that cause significant economic losses to agriculture worldwide. While feeding and probing these insects deliver molecules, called effectors, inside their host to enable infestation. The identification and characterization of these effectors from different species that vary in their host range is an important step in understanding the infestation success of aphids and aphid host range variation. This study employs a multi-disciplinary approach based on transcriptome sequencing and proteomics to identify and compare effector candidates from the broad host range aphid Myzus persicae (green peach aphid) (genotypes O, J and F), and narrow host range aphids Myzus cerasi (black cherry aphid) and Rhopalosiphum padi (bird-cherry oat aphid).Using a combination of aphid transcriptome sequencing on libraries derived from head versus body tissues as well as saliva proteomics we were able to predict candidate effectors repertoires from the different aphid species and genotypes. Among the identified conserved or core effector sets, we identified a significant number of previously identified aphid candidate effectors indicating these proteins may be involved in general infestation strategies. Moreover, we identified aphid candidate effector sequences that were specific to one species, which are interesting candidates for further validation and characterization with regards to species-specific functions during infestation. We assessed our candidate effector repertoires for evidence of positive selection, and identified 49 candidates with DN/DS ratios >1. We noted higher rates of DN/DS ratios in predicted aphid effectors than non-effectors. Whether this reflects positive selection due to co-evolution with host plants, or increased neofunctionalization upon gene duplication remains to be investigated.Our work provides a comprehensive overview of the candidate effector repertoires from three different aphid species with varying host ranges. Comparative analyses revealed candidate effectors that are most likely are involved in general aspects of infestation, whereas others, that are highly divergent, may be involved in specific processes important for certain aphid species. Insights into the overlap and differences in aphid effector repertoires are important in understanding how different species successfully infest different ranges of plant species.

Dataset Information

Comparative Proteomics of Sera From HCC Patients With Different Origins.

Publications

Comparative Proteomics of Sera From HCC Patients With Different Origins.

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