Dataset Information

Recognition of left-handed Z-DNA of short unmodified oligonucleotides under physiological ionic strength conditions.

ABSTRACT: The left-handed Z-DNA form of the short unmodified alternating guanine-cytosine oligonucleotides, 5'-(dGdC)(24) and 5'-(dGdC)(18), was selectively detected under physiological ionic strength and pH conditions using the anionic nickel(II) porphyrin, NiTPPS. No spectroscopic signal was observed for NiTPPS with any right-handed oligonucleotides under identical conditions. The 48mer 5'-(dGdC)(24) Z-form was detected at concentrations as low as 100nM. The binding of NiTPPS to the B- and Z-oligonucleotides was studied quantitatively by UV-vis absorption and circular dichroism spectroscopies. NiTPPS was found to be a universal DNA binder, with binding affinity and geometry depending on the ionic composition of the solution, rather than on the DNA helical twist. This is the first example of a successful spectroscopic detection of the Z-DNA of short unmodified oligonucleotides under physiological pH and ionic strength conditions.

SUBMITTER: D'Urso A

PROVIDER: S-EPMC3909744 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Recognition of left-handed Z-DNA of short unmodified oligonucleotides under physiological ionic strength conditions.

D'Urso Alessandro A Choi Jung Kyu JK Shabbir-Hussain Murtaza M Ngwa Fidelis N FN Lambousis Maria I MI Purrello Roberto R Balaz Milan M

Biochemical and biophysical research communications 20100527 2

The left-handed Z-DNA form of the short unmodified alternating guanine-cytosine oligonucleotides, 5'-(dGdC)(24) and 5'-(dGdC)(18), was selectively detected under physiological ionic strength and pH conditions using the anionic nickel(II) porphyrin, NiTPPS. No spectroscopic signal was observed for NiTPPS with any right-handed oligonucleotides under identical conditions. The 48mer 5'-(dGdC)(24) Z-form was detected at concentrations as low as 100nM. The binding of NiTPPS to the B- and Z-oligonucleo ...[more]

PMID: 20510880

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Dataset Information

Recognition of left-handed Z-DNA of short unmodified oligonucleotides under physiological ionic strength conditions.

Publications

Recognition of left-handed Z-DNA of short unmodified oligonucleotides under physiological ionic strength conditions.

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