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Transcriptional silencing by single-stranded RNAs targeting a noncoding RNA that overlaps a gene promoter.

ABSTRACT: RNAi using single-strand RNA would provide new options for therapeutic development and for investigating critical questions of mechanism. Using chemically modified single-strands, we test the hypothesis that single-stranded RNAs can engage the RNAi pathway and silence gene transcription. We find that a chemically modified single-stranded silencing RNA (ss-siRNA) designed to be complementary to a long noncoding RNA (lncRNA) requires argonaute protein, functions through the RNAi pathway, and inhibits gene transcription. These data expand the use of single-stranded RNA to cell nuclei.

SUBMITTER: Matsui M

PROVIDER: S-EPMC3909965 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Transcriptional silencing by single-stranded RNAs targeting a noncoding RNA that overlaps a gene promoter.

Matsui Masayuki M Prakash Thazha P TP Corey David R DR

ACS chemical biology 20121024 1

RNAi using single-strand RNA would provide new options for therapeutic development and for investigating critical questions of mechanism. Using chemically modified single-strands, we test the hypothesis that single-stranded RNAs can engage the RNAi pathway and silence gene transcription. We find that a chemically modified single-stranded silencing RNA (ss-siRNA) designed to be complementary to a long noncoding RNA (lncRNA) requires argonaute protein, functions through the RNAi pathway, and inhib ...[more]

PMID: 23082936

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