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In silico-prediction of downstream WRKY interacting partners of MAPK3 in Brassica.

ABSTRACT: Protein-Protein interactions (PPIs) are vital to most biological processes thus the identification of PPIs is of primary importance. Here, we endeavor to identify the downstream interacting partners of (BjMPK3P) in Brassica juncea using the docking approach. Out of 63 and 37 members of BrWRKY and BnWRKY transcription factors, 50 and 29 members are showing interactions with BjMPK3P respectively while the rest are showing non-interaction. Twenty two WRKY members are common to both the species. Using minimal sequence motif search as well as through docking approach several novel WRKY interacting proteins were also reported in the present study which need to be confirmed by in vitro kinase assay. Together, the results obtained essentially enhance our knowledge of the MAPK interacting protein network and provide a valuable research resource for developing a nearly important link between pathogen-activated MAPK signaling pathways and downstream transcriptional programming.

SUBMITTER: Giri P

PROVIDER: S-EPMC3910361 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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in silico-prediction of downstream WRKY interacting partners of MAPK3 in Brassica.

Giri Priyanka P Taj Gohar G Tasleem Mohd M Kumar Anil A

Bioinformation 20131227 20

Protein-Protein interactions (PPIs) are vital to most biological processes thus the identification of PPIs is of primary importance. Here, we endeavor to identify the downstream interacting partners of (BjMPK3P) in Brassica juncea using the docking approach. Out of 63 and 37 members of BrWRKY and BnWRKY transcription factors, 50 and 29 members are showing interactions with BjMPK3P respectively while the rest are showing non-interaction. Twenty two WRKY members are common to both the species. Usi ...[more]

PMID: 24497732

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Project description:The protein arginine methyl transferase PRMT5 is an enzyme expressed in oligodendrocyte lineage cells and responsible for the symmetric methylation of arginine residues on histone tails. Previous work from our laboratory identified PRMT5 as critical for myelination, due to its transcriptional regulation of genes involved in survival and early stages of differentiation. However, besides its nuclear localization, PRMT5 is found at high levels in the cytoplasm of several cell types, including oligodendrocyte progenitor cells (OPCs) and yet, its interacting partners in this lineage, remain elusive. By using mass spectrometry on protein eluates from extracts generated from primary oligodendrocyte lineage cells and immunoprecipitated with PRMT5 antibodies, we identified 1196 proteins as PRMT5 interacting partners. These proteins were related to molecular functions such as RNA binding, ribosomal structure, cadherin and actin binding, nucleotide and protein binding, and GTP and GTPase activity. We then investigated PRMT5 substrates using iTRAQ-based proteomics on cytosolic and nuclear protein extracts from CRISPR-PRMT5 knockdown immortalized oligodendrocyte progenitors compared to CRISPR-EGFP controls. This analysis identified a similar number of peptides in the two subcellular fractions and a total number of 57 proteins with statistically decreased symmetric methylation of arginine residues in the CRISPR-PRMT5 knockdown compared to control. Several PRMT5 substrates were in common with cancer cell lines and related to RNA processing, splicing and transcription. In addition, we detected ten oligodendrocyte lineage specific substrates, corresponding to proteins with high expression levels in neural tissue. They included: PRC2C, a proline-rich protein involved in methyl-RNA binding, HNRPD an RNA binding protein involved in regulation of RNA stability, nuclear proteins involved in transcription and other proteins related to migration and actin cytoskeleton. Together, these results highlight a cell-specific role of PRMT5 in OPC in regulating several other cellular processes, besides RNA splicing and metabolism.

Dataset Information

In silico-prediction of downstream WRKY interacting partners of MAPK3 in Brassica.

Publications

in silico-prediction of downstream WRKY interacting partners of MAPK3 in Brassica.

Similar Datasets

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