Ontology highlight
ABSTRACT:
SUBMITTER: Moses T
PROVIDER: S-EPMC3910630 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20140113 4
The saikosaponins comprise oleanane- and ursane-type triterpene saponins that are abundantly present in the roots of the genus Bupleurum widely used in Asian traditional medicine. Here we identified a gene, designated CYP716Y1, encoding a cytochrome P450 monooxygenase from Bupleurum falcatum that catalyzes the C-16α hydroxylation of oleanane- and ursane-type triterpenes. Exploiting this hitherto unavailable enzymatic activity, we launched a combinatorial synthetic biology program in which we com ...[more]