Ontology highlight
ABSTRACT:
SUBMITTER: Dong J
PROVIDER: S-EPMC3912567 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Analytical chemistry 20131213 1
In this work, we have developed a method that uses hydrogen-deuterium exchange (HDX) of C2-hydrogens of histidines coupled with mass spectrometry (MS) to identify Zn-bound histidines in metalloproteins. This method relies on differences in HDX reaction rates of Zn-bound and Zn-free His residues. Using several model peptides and proteins, we find that all Zn-bound His residues have substantially lower HDX reaction rates in the presence of the metal. The vast majority of non-Zn-binding His residue ...[more]