Ontology highlight
ABSTRACT:
SUBMITTER: Moon S
PROVIDER: S-EPMC3913590 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Moon Seunghyun S Siqueira-Neto Jair L JL Moraes Carolina Borsoi CB Yang Gyongseon G Kang Myungjoo M Freitas-Junior Lucio H LH Hansen Michael A E MA
PloS one 20140204 2
We present a customized high content (image-based) and high throughput screening algorithm for the quantification of Trypanosoma cruzi infection in host cells. Based solely on DNA staining and single-channel images, the algorithm precisely segments and identifies the nuclei and cytoplasm of mammalian host cells as well as the intracellular parasites infecting the cells. The algorithm outputs statistical parameters including the total number of cells, number of infected cells and the total number ...[more]