Noncanonical K27-linked polyubiquitination of TIEG1 regulates Foxp3 expression and tumor growth.

ABSTRACT: Earlier, we demonstrated the essential role of Kruppel-like transcription factor, TIEG1, in TGF-?-induced regulatory T cell (Treg) development. In this article, we demonstrate that IL-6, which promotes Th17 development, abrogated TIEG1 nuclear translocation and inhibited TGF-?-induced Treg development. Tyrosine kinase Tyk2-mediated phosphorylation of TIEG1 at Tyr179 promoted noncanonical K-27-linked polyubiquitination, which inhibited TIEG1 nuclear translocation. To test the role of TIEG1-regulated Treg/Th17 development in antitumor immunity, we analyzed TRAMP-C2 tumor growth in TIEG1(-/-) mice. The defective Treg development and elevated Th17 response resulted in enhanced immune reactivity in the tumor and inhibition of TRAMP-C2 tumor growth in TIEG1(-/-) mice. Thus, our results uncovered a novel regulatory mechanism that modulates Tregs and may regulate tumor progression.

SUBMITTER: Peng DJ 

PROVIDER: S-EPMC3914214 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature