Unknown

Dataset Information

0

Analysis of high pI ?-Amy-1 gene family members expressed in late maturity ?-amylase in wheat (Triticum aestivum L.).

ABSTRACT: Late maturity ?-amylase (LMA) is a genetic defect involving the synthesis of high pI isozymes of ?-amylase encoded by ?-Amy-1 genes during the later stages of grain development. The aims of this investigation were to determine both the number of expressed ?-Amy-1 genes and their relative transcript abundance. Sub-cloning and sequencing of expressed high pI ?-amylase genes in developing wheat seeds revealed three insertion/deletion patterns in the 3' untranslated region and numerous single nucleotide polymorphisms at the 3' end of ?-Amy-1. The genetic variations defined 36 ?-Amy-1 gene sequences that were expressed on the onset of LMA in doubled haploid progenies (SpM25, SpM52 and SpM127) derived from the cross Spica (LMA)/Maringa (non-LMA). Five isoelectric point groups were predicted based on the translated partial coding sequences. The potential application of quantitative real-time RT-PCR in screening wheat genotypes for LMA is discussed.

SUBMITTER: Cheng CR 

PROVIDER: S-EPMC3918125 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Analysis of high pI <i>α</i>-<i>Amy</i>-<i>1</i> gene family members expressed in late maturity α-amylase in wheat (<i>Triticum aestivum</i> L.).

Cheng Cong-Rong CR   Oldach Klaus K   Mrva Kolumbina K   Mares Daryl D  

Molecular breeding : new strategies in plant improvement 20131017

Late maturity α-amylase (LMA) is a genetic defect involving the synthesis of high pI isozymes of α-amylase encoded by <i>α</i>-<i>Amy</i>-<i>1</i> genes during the later stages of grain development. The aims of this investigation were to determine both the number of expressed <i>α</i>-<i>Amy</i>-<i>1</i> genes and their relative transcript abundance. Sub-cloning and sequencing of expressed high pI α-amylase genes in developing wheat seeds revealed three insertion/deletion patterns in the 3' untr  ...[more]

Similar Datasets

Unknown Does Late Maturity Alpha-Amylase Impact Wheat Baking Quality?
| S-EPMC6137811 | biostudies-literature
Transcriptomics Wheat (Triticum aestivum L.) transcriptome profiling
2014-12-31 | GSE58805 | GEO
Unknown Comprehensive functional analyses of expressed sequence tags in common wheat (Triticum aestivum).
| S-EPMC3325080 | biostudies-literature
Unknown Gibberellins in developing wheat grains and their relationship to late maturity α-amylase (LMA).
| S-EPMC9076747 | biostudies-literature
Unknown Enzymic capacities of amyloplasts from wheat (Triticum aestivum) endosperm.
| S-EPMC1135240 | biostudies-other
Transcriptomics Ultrasound effect on the wheat (Triticum aestivum) seedlings
2022-09-14 | GSE200377 | GEO
Unknown The amino acid sequence of ferredoxin from Triticum aestivum (wheat).
| S-EPMC1186635 | biostudies-other
Unknown Proteomic analysis of middle and late stages of bread wheat (Triticum aestivum L.) grain development.
| S-EPMC4569854 | biostudies-literature
Unknown Global identification and characterization of miRNA family members responsive to potassium deprivation in wheat (Triticum aestivum L.).
| S-EPMC7519128 | biostudies-literature
Transcriptomics Wheat (Triticum aestivum L.) transcriptome profiling
2014-12-31 | E-GEOD-58805 | biostudies-arrayexpress