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Analysis of high pI ?-Amy-1 gene family members expressed in late maturity ?-amylase in wheat (Triticum aestivum L.).

ABSTRACT: Late maturity ?-amylase (LMA) is a genetic defect involving the synthesis of high pI isozymes of ?-amylase encoded by ?-Amy-1 genes during the later stages of grain development. The aims of this investigation were to determine both the number of expressed ?-Amy-1 genes and their relative transcript abundance. Sub-cloning and sequencing of expressed high pI ?-amylase genes in developing wheat seeds revealed three insertion/deletion patterns in the 3' untranslated region and numerous single nucleotide polymorphisms at the 3' end of ?-Amy-1. The genetic variations defined 36 ?-Amy-1 gene sequences that were expressed on the onset of LMA in doubled haploid progenies (SpM25, SpM52 and SpM127) derived from the cross Spica (LMA)/Maringa (non-LMA). Five isoelectric point groups were predicted based on the translated partial coding sequences. The potential application of quantitative real-time RT-PCR in screening wheat genotypes for LMA is discussed.

SUBMITTER: Cheng CR 

PROVIDER: S-EPMC3918125 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Analysis of high pI <i>α</i>-<i>Amy</i>-<i>1</i> gene family members expressed in late maturity α-amylase in wheat (<i>Triticum aestivum</i> L.).

Cheng Cong-Rong CR   Oldach Klaus K   Mrva Kolumbina K   Mares Daryl D  

Molecular breeding : new strategies in plant improvement 20131017

Late maturity α-amylase (LMA) is a genetic defect involving the synthesis of high pI isozymes of α-amylase encoded by <i>α</i>-<i>Amy</i>-<i>1</i> genes during the later stages of grain development. The aims of this investigation were to determine both the number of expressed <i>α</i>-<i>Amy</i>-<i>1</i> genes and their relative transcript abundance. Sub-cloning and sequencing of expressed high pI α-amylase genes in developing wheat seeds revealed three insertion/deletion patterns in the 3' untr  ...[more]

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