Colorectal carcinomas with CpG island methylator phenotype 1 frequently contain mutations in chromatin regulators.

ABSTRACT:

Background & aims

Subgroups of colorectal carcinomas (CRCs) characterized by DNA methylation anomalies are termed CpG island methylator phenotype (CIMP)1, CIMP2, or CIMP-negative. The pathogenesis of CIMP1 colorectal carcinomas, and their effects on patients' prognoses and responses to treatment, differ from those of other CRCs. We sought to identify genetic somatic alterations associated with CIMP1 CRCs.

Methods

We examined genomic DNA samples from 100 primary CRCs, 10 adenomas, and adjacent normal-appearing mucosae from patients undergoing surgery or colonoscopy at 3 tertiary medical centers. We performed exome sequencing of 16 colorectal tumors and their adjacent normal tissues. Extensive comparison with known somatic alterations in CRCs allowed segregation of CIMP1-exclu

SUBMITTER: Tahara T 

PROVIDER: S-EPMC3918446 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature