Ontology highlight
ABSTRACT:
SUBMITTER: Chandler BD
PROVIDER: S-EPMC3919123 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Chandler Brent D BD Burkhardt Anne L AL Foley Klaudia K Cullis Courtney C Driscoll Denise D Roy D'Amore Natalie N Miller Scott J SJ
Journal of the American Chemical Society 20131217 1
We report the synthesis and biochemical validation of a phosphatidyl inositol-3 phosphate (PI3P) immunogen. The inositol stereochemistry was secured through peptide-catalyzed asymmetric phosphorylation catalysis, and the subsequent incorporation of a cysteine residue was achieved by native chemical ligation (NCL). Conjugation of the PI3P hapten to maleimide-activated keyhole limpet hemocyanin (KLH) provided a PI3P immunogen, which was successfully used to generate selective PI3P antibodies. The ...[more]