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Noninvasive positron emission tomography and fluorescence imaging of CD133+ tumor stem cells.


ABSTRACT: A technology that visualizes tumor stem cells with clinically relevant tracers could have a broad impact on cancer diagnosis and treatment. The AC133 epitope of CD133 currently is one of the best-characterized tumor stem cell markers for many intra- and extracranial tumor entities. Here we demonstrate the successful noninvasive detection of AC133(+) tumor stem cells by PET and near-infrared fluorescence molecular tomography in subcutaneous and orthotopic glioma xenografts using antibody-based tracers. Particularly, microPET with (64)Cu-NOTA-AC133 mAb yielded high-quality images with outstanding tumor-to-background contrast, clearly delineating subcutaneous tumor stem cell-derived xenografts from surrounding tissues. Intracerebral tumors as small as 2-3 mm also were clearly discernible, and the microPET images reflected the invasive growth pattern of orthotopic cancer stem cell-derived tumors with low density of AC133(+) cells. These data provide a basis for further preclinical and clinical use of the developed tracers for high-sensitivity and high-resolution monitoring of AC133(+) tumor stem cells.

SUBMITTER: Gaedicke S 

PROVIDER: S-EPMC3926065 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

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Noninvasive positron emission tomography and fluorescence imaging of CD133+ tumor stem cells.

Gaedicke Simone S   Braun Friederike F   Prasad Shruthi S   Machein Marcia M   Firat Elke E   Hettich Michael M   Gudihal Ravindra R   Zhu Xuekai X   Klingner Kerstin K   Schüler Julia J   Herold-Mende Christel C CC   Grosu Anca-Ligia AL   Behe Martin M   Weber Wolfgang W   Mäcke Helmut H   Niedermann Gabriele G  

Proceedings of the National Academy of Sciences of the United States of America 20140127 6


A technology that visualizes tumor stem cells with clinically relevant tracers could have a broad impact on cancer diagnosis and treatment. The AC133 epitope of CD133 currently is one of the best-characterized tumor stem cell markers for many intra- and extracranial tumor entities. Here we demonstrate the successful noninvasive detection of AC133(+) tumor stem cells by PET and near-infrared fluorescence molecular tomography in subcutaneous and orthotopic glioma xenografts using antibody-based tr  ...[more]

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