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Protein-engineered injectable hydrogel to improve retention of transplanted adipose-derived stem cells.

ABSTRACT: Improved retention of transplanted stem cells is achieved through minimally invasive delivery in MITCH, a mixing-induced two-component hydrogel that was engineered to possess shear-thinning and self-healing thixotropic properties. MITCH, an ideal injectable cell-delivery vehicle, supports 3D stem-cell culture, resulting in high cell viability and physiologically relevant cell morphology.

SUBMITTER: Parisi-Amon A

PROVIDER: S-EPMC3926432 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature

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Protein-engineered injectable hydrogel to improve retention of transplanted adipose-derived stem cells.

Parisi-Amon Andreina A Mulyasasmita Widya W Chung Cindy C Heilshorn Sarah C SC

Advanced healthcare materials 20120928 3

Improved retention of transplanted stem cells is achieved through minimally invasive delivery in MITCH, a mixing-induced two-component hydrogel that was engineered to possess shear-thinning and self-healing thixotropic properties. MITCH, an ideal injectable cell-delivery vehicle, supports 3D stem-cell culture, resulting in high cell viability and physiologically relevant cell morphology. ...[more]

PMID: 23184882

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Project description:BackgroundStem cell therapy has revealed a promising future for treating erectile dysfunction (ED), but the fate and curative mechanism of intracavernosal transplanted stem cells are under further exploration. This study aimed to demonstrate the effects of myocardin gene modification on improving erectile function and prolonging the retention of implanted adipose-derived stem cells (ASCs) using in vivo small animal imaging.MethodsASCs were isolated, cultured, and identified by flow cytometry and osteogenic and adipogenic induction. The effects of gene modification on cell proliferation, apoptosis, and contraction were determined by CCK-8, EdU, flow cytometry, and collagen gel lattice contraction assays as well as confocal microscopy. A total of 20 normal and 60 diabetes mellitus ED to (DMED) Sprague-Dawley rats were recruited to the 7?day and 21?day groups. Each group contained subgroups of 10 rats each: the negative control (NC), DMED + ASCs plus Ad-Luc-Myocardin, DMED + ASCs plus Ad-Luc, and DMED + phosphate buffer solution (PBS) groups. Erectile function was evaluated with the intracavernosal pressure/mean arterial pressure (?ICP/MAP) ratio. In vivo small animal imaging and an EdU cell tracking strategy were introduced to detect the transplanted ASCs, and IHC and WB were performed to assess smooth muscle cell protein levels.ResultsThe ASCs expressed high CD29 and CD90 and scant CD45, while the multi-induction potential was verified by oil red O and alizarin red staining. Gene transfection of myocardin had no significant influence on ASC apoptosis but inhibited cell proliferation and promoted cell contraction. Myocardin combined with ASCs enhanced the therapeutic potential of ASCs for improving the ?ICP/MAP ratio as well as ?-SMA and calponin expression. In vivo imaging confirmed that ASCs resided within the cavernous body in 21?days, while only a few red EdU dots were detected.ConclusionsMyocardin induced ASC differentiation towards smooth muscle-like cells and enhanced the therapeutic potential of ASCs for ameliorating ED in STZ-induced diabetic rats. Notably, in vivo small animal tracking was an effective strategy for monitoring the implanted stem cells, and this strategy might have advantages over traditional EdU assays.

Dataset Information

Protein-engineered injectable hydrogel to improve retention of transplanted adipose-derived stem cells.

Publications

Protein-engineered injectable hydrogel to improve retention of transplanted adipose-derived stem cells.

Similar Datasets

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