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Endoglin is necessary for angiogenesis in human ovarian carcinoma-derived primary endothelial cells.

ABSTRACT: Endoglin (CD105, END) is upregulated in proliferating endothelial cells, suggesting potential therapeutic properties. However, it is not clear whether endoglin mediates an enhanced proliferative rate or may be upregulated as part of a negative feedback loop. To gain insights into context-dependent and cell type-dependent regulatory effects of endoglin, we studied its role properties in human ovarian carcinoma-derived endothelial cells (ODMECs). We isolated and cultured primary ODMECs from epithelial ovarian carcinoma tissue. ODMECs had higher expression of endoglin and VEGFR-2, and also exhibited enhanced spontaneous formation of vessel-like structures in vitro. Transfection of siRNA targeting endoglin in ODMECs cells resulted in the reduction of the proliferation and tube formation. These results indicate that a subset of ODMECs display abnormal angiogenic properties and this phenotype was blocked by decreasing endoglin levels, suggesting endoglin is essential for stimulating angiogenesis, and targeting it may be an attractive approach to anti-angiogenesis therapy for ovarian carcinoma.

SUBMITTER: Xu Y

PROVIDER: S-EPMC3926891 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Endoglin is necessary for angiogenesis in human ovarian carcinoma-derived primary endothelial cells.

Xu Yan Y Wang Dan D Zhao Li-Mei LM Zhao Xi-Long XL Shen Jun-Jie JJ Xie Yao Y Cao Li-Li LL Chen Zhen-Bo ZB Luo Yan-Mei YM Bao Bi-Hui BH Liang Zhi-Qing ZQ

Cancer biology & therapy 20130805 10

Endoglin (CD105, END) is upregulated in proliferating endothelial cells, suggesting potential therapeutic properties. However, it is not clear whether endoglin mediates an enhanced proliferative rate or may be upregulated as part of a negative feedback loop. To gain insights into context-dependent and cell type-dependent regulatory effects of endoglin, we studied its role properties in human ovarian carcinoma-derived endothelial cells (ODMECs). We isolated and cultured primary ODMECs from epithe ...[more]

PMID: 23917399

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Dataset Information

Endoglin is necessary for angiogenesis in human ovarian carcinoma-derived primary endothelial cells.

Publications

Endoglin is necessary for angiogenesis in human ovarian carcinoma-derived primary endothelial cells.

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